Visualising and understanding the control of chromatin organisation
Dr Abder Kaidi, University of Bristol
Date & Time:
Tuesday, 14 March 2017, 11:00
Re-establishment of nuclear structure and chromatin organisation after cell division is integral for genome regulation and cell function. However, the mechanisms underlying this process remain incompletely understood. To address this, we developed imaging approaches to visualise chromatin organisation in intact cells. These include: florescence lifetime imaging microscopy (FLIM), florescence correlation spectroscopy (FCS), and electron microscopy. Thus, we identify a new role for nuclear actin in organising chromatin after mitosis. Interference with this function influences transcription and replication in the daughter cells. These findings identify a novel spatiotemporally controlled form of nuclear actin that contributes to the chromatin organization after cell division. This has implications for understanding the mechanisms underlying the overall organisation of the mammalian somatic cell nucleus.